Multi Parametric Surface Plasmon Resonance System (MP-SPR) for Materials and Life Science

The Life Science perspective: From traditional SPR to Multi-Parametric SPR – from measurements to understanding.

Surface Plasmon Resonance (SPR) is an established method for biomolecular interaction analysis. It is popular due to its sensitivity and its real-time label-free principle. Multi-Parametric Surface Plasmon Resonance (MP-SPR) is based on SPR theory, however its advantageous optical setup measures a full SPR curve which enables new insight into interactions. For instance, PureKinetics™ feature that provides measurements of small molecules, lipids and biomaterials without bulk effect (typical DMSO artefact). MP-SPR widens the application range of traditional SPR from small molecules up to nanoparticles and even living cells. Measurements can be performed even in complex media such as serum.

MP-SPR uniquely provides also information about layer properties. Thickness and refractive index (RI) data can be utilized in material characterization from Ångström thick layers up to micrometers or to ensure conformation of the molecules on the surface.

The Material Science perspective: From drug discovery to Ångström precision in coatings and material development.

The key to the Multi-Parametric Surface Plasmon Resonance is the measurement of full SPR curves. When measured as a function of time (with sampling rate of one curve every few seconds), the results can be calculated to many different physical parameters describing the sample properties or interactions.

  • True thickness: Thickness and Refractive index (RI) can be determined by fitting of curves using Fresnel formalism. With additional lasers, a singular solution can be found without known RI or thickness.
  • From Å to µm: Unique wide angle range measurement enables measurement not only of thin layers (Ångströms) but also thicker layers up to a few micrometers.
  • No vacuum required: The method allows measurements of samples in air, in a specific gas, humidity or in liquid environment.
  • Real-time interactions: MP-SPR is a real-time method and therefore, allows for instance measurements of swelling of materials when moving from dry to wet environment. It also provides real-time data on material-solvent interactions, for instance